Validation of HPLC Determination of 1-Hydroxypyrene in Urine

Authors

  • I. Bajusová Department of Occupational Medicine and Clinical Toxicology, Faculty of Medicine, P. J. Šafárik University, Košice and L. Pasteur University Hospital, Košice, Slovakia
  • Ľ. Legáth Department of Occupational Medicine and Clinical Toxicology, Faculty of Medicine, P. J. Šafárik University, Košice and L. Pasteur University Hospital, Košice, Slovakia

Keywords:

polycyclic aromatic hydrocarbons, 1-hydroxypyrene, HPLC, urine

Abstract

Exposure to polycyclic aromatic hydrocarbons (PAH) is a serious hazard for human health. 1-Hydroxypyrene (1‑HOP), a major metabolite of pyrene, is used as a bio­marker for the assessment of an exposure to PAH. The article deals with the validation of the HPLC determination of 1-HOP with fluorescence detection. 1-HOP was isolated from urine by solid phase extraction. The detection and quantification limits are 0.08 and 0.26 µg l–1, respectively. RSD of precision and accuracy are lower than 9.8 %. The recoveries are 78.8–93.5 %. This method can be used to determine and monitor the urinary 1-HOP in workers exposed to PAH.

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Published

2015-01-15

How to Cite

Bajusová, I., & Legáth, Ľ. (2015). Validation of HPLC Determination of 1-Hydroxypyrene in Urine. Chemické Listy, 109(1), 55–60. Retrieved from http://w-ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/415

Issue

Vol. 109 No. 1 (2015)

Section

Articles