Determination of Silybin in Blood Plasma Using High Performance Liquid Chromatography with Solid Phase Extraction

Authors

  • P. Kosina Centre for Bioanalytical Research, Palacky University,
  • J. Bartek Institute of Medical Chemistry and Biochemistry, Medical Faculty , Palacky University, Olomouc

Abstract

HPLC method with SPE preseparation step for determination of silybin, the main constituent of silymarin, in blood plasma was optimized. A C18 sorbent for preseparation from acidified samples was used. Diastereomers of silybin in unconjugated form or total silybin are resolved by the method, the detection limit being 2 nmol/l and the limit of quantification 5 nmol/l per diastereomer. The method is suitable for silybin monitoring in human plasma after administration of drugs or food additives based on silymarin.

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Published

2001-01-15

How to Cite

Kosina, P., & Bartek, J. (2001). Determination of Silybin in Blood Plasma Using High Performance Liquid Chromatography with Solid Phase Extraction. Chemické Listy, 94(12). Retrieved from http://w-ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2464

Issue

Vol. 94 No. 12 (2000)

Section

Articles