A Method of Analysis of Isoprostanes in Salmon Tissue

Authors

  • M. Bolechová Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Technical University, Brno, Czech Republic | Central Inspectional and Testing Institute in Agriculture, Brno, Czech Republic
  • J. Čáslavský Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Technical University, Brno, Czech Republic
  • D. Mácová Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Technical University, Brno, Czech Republic
  • M. Vávrová Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Technical University, Brno, Czech Republic
  • M. Taggart Environmental Research Institute, North Highland College, Thurso, UK

Keywords:

oxidative stress, mass spectrometry, liquid chromatography, isoprostanes

Abstract

A sensitive and reliable method is proposed allowing quantification of isoprostanes in salmon tissue using ultra-HPLC coupled to tandem MS. Solid phase extraction is used for sample concentration and purification. Repeatability, linearity, limits of detection and quantification were assessed by validation.

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Published

2015-04-15

How to Cite

Bolechová, M., Čáslavský, J., Mácová, D., Vávrová, M., & Taggart, M. (2015). A Method of Analysis of Isoprostanes in Salmon Tissue. Chemické Listy, 109(4), 305–312. Retrieved from http://w-ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/385

Issue

Vol. 109 No. 4 (2015)

Section

Articles