Using a Commercial Method for Rubisco Immunodepletion in Analysis of Plant Proteom

Authors

  • Martin Černý Laboratory of Molecular Plant Biology, Mendel University in Brno and Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno
  • Jan Skalák Laboratory of Molecular Plant Biology, Mendel University in Brno and Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno
  • Barbora Kurková Laboratory of Molecular Plant Biology, Mendel University in Brno and Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno
  • Elena Babuliaková Laboratory of Molecular Plant Biology, Mendel University in Brno and Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno
  • Břetislav Brzobohatý Laboratory of Molecular Plant Biology, Mendel University in Brno and Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno

Keywords:

plant proteomics, Rubisco, 2D electrophoresis, proteome enrichment

Abstract

Rubisco immunodepletion was tested using a Seppro IgY-Rubisco spin column in 2D-PAGE-based proteomic analysis. The column yield was analyzed in 20 consecutive runs and the depletion from tissues with higher and lower Rubisco contents was evaluated. An increase in the number of detected protein spots of up to 25 % was found. This procedure allows to follow the profiling of a similar number of protein spots in Rubisco-depleted proteom resolved on small gels as is usual with standard total protein extracts resolved on large gels. We found this procedure especially suitable for experiments with a limited amount of the starting material.

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Published

2011-08-15

How to Cite

Černý, M., Skalák, J., Kurková, B., Babuliaková, E., & Brzobohatý, B. (2011). Using a Commercial Method for Rubisco Immunodepletion in Analysis of Plant Proteom. Chemické Listy, 105(8), 640–642. Retrieved from http://w-ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1103

Issue

Vol. 105 No. 8 (2011)

Section

Articles