Development of a Non-Invasive Diagnostic Method for Bronchial Asthma

Autoři

  • K. Syslová Department of Organic Technology, Institute of Chemical Technology, Prague,
  • J. Přech Department of Organic Technology, Institute of Chemical Technology, Prague,
  • J. Lebedová Department of Occupational Medicine, 1st Faculty of Medicine, Charles University, Prague
  • D. Pelclová Department of Occupational Medicine, 1st Faculty of Medicine, Charles University, Prague
  • P. Kačer Department of Organic Technology, Institute of Chemical Technology, Prague,

Abstrakt

A method was developed for rapid and accurate determination of cysteinylleukotrienes (LTC4, LTD4, LTE4), essential biomarkers of bronchial asthma, in exhaled breath condensate. The method consists of an immunoseparation procedure combined with selective and sensitive detection by LC-ESI-MS in the multiple reaction monitoring mode (MRM) and using a stable-isotope-dilution assay. The precision of the method was around 94 %. The mean accuracy was higher than 90 % for the content of cysteinylleukotrienes in the concentrate up to 250 pg ml-1. The method was tested on patients with occupational bronchial asthma and compared with healthy subjects. The differences between the two groups were significant.

Stahování

Publikováno

15.04.2009

Jak citovat

Syslová, K., Přech, J., Lebedová, J., Pelclová, D., & Kačer, P. (2009). Development of a Non-Invasive Diagnostic Method for Bronchial Asthma. Chemické Listy, 103(3). Získáno z http://w-ww.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1562

Číslo

Vol 103 No 3 (2009)

Sekce

Články